Structural Biology Group

Projects

Our research is related to the characterization of the so-called structure-function-relationship of bio-macromolecules. For the determination of the three-dimensional structures we are using single crystal X-ray diffraction. For the functional characterization of the enzymatic activity and/or complex formation with other bio-macromolecules we make use of different biophysical methods like affinity chromatography, fluorescence spectroscopy, surface plasmon resonance spectroscopy, light scattering and mass spectrometry. In our laboratory we have access to all equipment needed for molecular biology (PCR, sequencing, gel imaging), expression (10 l fermentation and different incubators), purification (different Äkta systems and HPLC), crystallization (Mosquito), X-ray data collection (MarµX) and structure refinement. We take advantage of molecular- and cell biological techniques to characterize the function and regulation of our target proteins. This includes enzymatic activity assays, flow cytometry, fluorescence microscopy, co-immunoprecipitation, cell surface biotinylation and Western blotting. Beside endogen expression we modify expression levels by overexpression or by downregulation in different eukaryotic cell lines.

Our research topics are related to

  • Signal transduction
  • Biotransformation
  • Metabolism
  • Kinetic and time-resolved protein crystallography
  • Sensory proteins
  • Extracellular oxidoreduktases
  • Thiol-Switches
  • Regulation of ectodomain shedding